Person: ATAK, ÇİMEN
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ATAK
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ÇİMEN
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Publication Embargo Micropropagation of Anthurium adreanum from leaf explants(Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Kultur University, Ataköy, Istanbul, Turkey, 2009) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Anthurium propagation by tissue culture techniques appears as an alternative to increase the production. Plant regeneration of Anthurium andraeanum cultivars “Arizona” and “Sumi” (Anthura) has been achieved through adventitious shoots formation from callus. The leaf explants were used for the propagation by indirect organogenesis. Callus induction medium consisted of half-strength MS basal salt with 0.6 mg/l 2,4-D, 1 mg/l BA. Shoot regeneration medium consisted of modified halfstrength MS salts with NH4NO3 lowered to 250 mg/l, 0.1 mg/l 2, 4-D and 1 mg/l BA. Rooting medium was (modified half-strength MS salts) supplemented with 1mg/l IBA and 0.04%active charcoals. The callus induction rate of Arizona variety was higher as compared to Sumi variety and this increase was found significant. The number of shoots per explants for Arizona variety also significantly increased as compared to Sumi variety. Arizona and Sumi root percentages were high and these percentages were 98% and 95% respectively. The results showed that Arizona variety had different responses as compared to Sumi variety for callus induction and the number of shoots per explants. Shoot multiplication for two varieties increased in the multiplication stages and this increase in number of shoots per explants were found important (p<0.05).Publication Metadata only Effect of magnetic field on peroxidase activities of soybean tissue culture(DIAGNOSIS PRESS LTD, 67 DONDUKOV BLVD, 1504 SOFIA, BULGARIA, 2007-05) Olgun, Atok; Alikamanoğlu, Sema; Rzakoulieva, A.; ATAK, ÇİMEN; ÇELİK, ÖZGE; TR6653; TR141172In this study, the aim was to determine the effect of magnetic field on peroxidase activities of soybean tissue culture. Shoot tips were put into petri dishes and exposed to a magnetic field for a period of 2.2 and 19.8 s at a magnetic flux of 2.9-4.6 mT. The shoot and root formation rate, fresh weights, chlorophyll quantities, total RNA concentrations and peroxidase activities of regenerated shoots from control and treated shoot tips were determined. While the rate of shoot formation was 28.57% in the control group, this rate was increased to 94.33% and 78.18%, respectively, in the explants that were exposed to a magnetic field for a period of 2.2 and 19.8 s. While the percentage of root formation in controls was 4.76%, this rate increased to 47.17% and 54.54%, respectively, in those that were exposed to a magnetic field at the same periods. When the fresh weights were determined, we found that the fresh weights of plantlets regenerated from treated explants were increased relative to controls. Chlorophyll a, chlorophyll b and total chlorophyll contents increased 21%, 13% and 18%, respectively, relative to control groups at 2.2 s. Peroxidase activity significantly increased in all magnetic field treatments (p<0.05). The total RNA concentration of seedlings regenerated from treatment explants significantly increased relative to controls (p<0.05). The regeneration and plant growth of shoot tips exposed to a magnetic field with a 2.2 s period were positively affected by the MF and increased with respect to controls and the length of time exposed.Publication Metadata only Effect of magnetic field on Paulownia seeds(2000) Yurttaş, B.; Alikamanoğlu, S.; Topçul, F.; Rzakoulieva, A.; Danilov, V.; ATAK, ÇİMEN; 6653Publication Metadata only In Vitro and In Vivo Investigation of Salt Tolerance in Two Oriental Tobacco (Nicotiana tabacum L.) Species.(Springer, 233 Spring St, New York, Ny 10013 USA, 2009-09) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Publication Metadata only Induced of plastid mutations in soybean plant (Glycine max L. Merrill) with gamma radiation and determination with RAPD(2004-11-22) Alikamanoğlu, Sema; Açık, Leyla; Canpolat, Yasemin; ATAK, ÇİMEN; 6653; 176641; 1781The aim of our study was to induce with radiation of atrazine resistant and tolerated mutants in Coles, Amsoy-71 and 1937 soybean varieties. Atrazine that is photosynthetic inhibitor is the most important herbicide of S-triazin group, and shows toxic effect on soybean plant. For the improvement of the atrazine resistant plants with mutation breeding, the seeds belonging to the three varieties were irradiated with 200 Gy of gamma radiation dose. The irradiated seeds were sown in the field and at the end of harvesting season, every pod at node situated on the main stem was picked up separately and M2 generations were obtained. At the plants, which were obtained from M2 generation, chlorophyll mutants were determined and atrazine selection was made. The percentage of chlorophyll mutants for Amsoy-71, Coles and 1937 soybean varieties were found as 1.07, 1.48 and 1.32, respectively. At the end of atrazine selection, the percentages of atrazine resistant plants for Amsoy-71, Coles and 1937 soybean varieties were 0.80, 0.60 and 0.53, respectively. The percentages of atrazine tolerated plants were 1.07, 1.18 and 1.05, respectively as well. In our research; the differences among the mutants replying to atrazine in various concentrations were examined by using RAPD procedure as the molecular marker techniques in comparison with polymorphism. In the study done by using 14 primers; according to the amplification results, the differences between atrazine resistant plants were shown.Publication Embargo Effect of magnetic field on L-strain cells(2000) Ulakoğlu, G.; Yurttaş, B.; Rzakoulieva, A.; Danilov, V.; ATAK, ÇİMEN; 6653Publication Metadata only Role of Meta topalin in Tissue Culture of Arabidopsis thaliana(2009) Ünsal, Zeynep Narçın; Büyüktuncer, Damla; ATAK, ÇİMEN; 6125; 6653Publication Embargo The effect of magnetic field on the activity of superoxide dismutase(Haliç University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Fındıkzade 34280, İstanbul-Turkey, 2006) Büyükuslu, Nihal; ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987; 109872The effects of magnetic field on superoxide dismutase activity were investigated. All living systems are affected by magnetic field and electromagnetic field in a way of their response systems. Since magnetic field has an impact on biochemical reactions that involve more than one unpaired electron, in our study SOD activity, one of the enzyme responsible for antioxidant system, was measured under magnetic fields using an apparatus explained at material methods. There has been a significant increase of SOD activity when passed 0, 1, 9 and 15 times at 2.9-4.6 mT magnetic field density for 0, 2.2, 19.8 and 33.0 seconds respectively.Publication Metadata only The effects of magnetic field on germination of soybean seeds and on the activity of superoxide dismutase and catalase(Springer, 233 Spring St, New York, Ny 10013 USA, 2007-09) Büyükuslu, Nihal; ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987; 109872Publication Metadata only Effect of magnetic field on Paulownia seeds(2000) Yurttaş, B.; Alikamanoğlu, Sema; Topçul, F.; Rzakoulieva, Aytekin; Danilov, V.; ATAK, ÇİMEN; 6653; 176641