Person: ATAK, ÇİMEN
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ATAK
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ÇİMEN
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Publication Metadata only Selection and Molecular Profiling Of Salt Tolerant Tobacco Plants Obtained By Gamma Radiation(2010) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Publication Metadata only Effects of Salinity Stress on Densities of Trichomes, Glandular Trichomes, and Stomata in Two Soybean Varieties(Springer, 233 Spring St, New York, Ny 10013 USA, 2010-09) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 4019; 6653Publication Embargo Micropropagation of Anthurium spp(InTech Open Science, 2012) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Publication Metadata only Differential regulation of antioxidative gene expressions in response to salt stress in rice(International Congress on Transcriptomics 27-29 Temmuz 2015, 2015) Candar, Bilgin; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 195745; 6653Rice (Oryza sativa L.) is one of the most important crops because it is a nutritional source of more than one-third of the world population. Soil salinity is one of the most important abiotic stress factors that affect plant growth and productivity adversely. Rice growth and yield is also affected by salinity and at the seedling stage although it is known to be susceptible to salinity. Reactive oxygen species are induced by salt stress and some responsive mechanisms are evolved against to the detrimental effects caused by salt stress. Salinity response is especially controlled by obtaining homeostasis between antioxidative mechanisms and accumulation of reactive oxygen species (ROS) produced as a result of oxidative stress caused by salinity. It is known that combined expression profiles of antioxidative system enzymes may provide increased tolerance capacity. Therefore, the expression profiles of antioxidative enzymes in two different rice cultivars which have different salt sensitivities at four different salt stress conditions were determined. Salinity responses of two different rice varieties were investigated at seedling stage. Four different salinity treatments were then applied using Yoshida solution containing 0, 30, 90, 150 and 210 mM NaCl to the nutrient solution for seven days. At the end of the seven days, the leaves were harvested and stored at -20oC for further experiments. The salt stress responsive gene specific primers were amplified by designed primers due to the 3?-UTR regions of each of the following genes by aligning all available related genes in the databases of NCBI and KOME: Mn-SOD, Cu/Zn-SOD, Fe-SOD, Cytosolic APX, Thylakoid-bound APX, stromal APX, Cytosolic GR and CatA. Mn-SOD was consisted with the trend of variation in SOD activities of rice varieties. The expression patternof CAT A gene was markedly decreased compared to control in both rice varieties. Str-APX gene expression was up-regulated during salt stress treatments in both rice varieties. Transcript levels of Cyt-APX and Thy-APX were up-regulated in accordance with increasing salt stress in Osmanc?k-97 variety. The expression pattern of gene encoding enzyme Cyt-GR1 showed a gradual up-regulation as a response to subjected increasing NaCl stress in Mevl�tbey variety while only after 90 mM treatment, an up-regulation was observed for Osmanc?k variety. These data indicated that the antioxidative responses of salt tolerant and salt sensitive rice varieties are differentially regulated.Publication Metadata only FTIR and EDXRF Investigations of Salt Tolerant Soybean Mutants(Elsevier Science Bv, Po Box 211, 1000 Ae Amsterdam, Netherlands, 2013-07-23) AKYÜZ, SEVİM; AKYÜZ, ZEKİ TANIL; ATAK, ÇİMEN; ÇELİK, ÖZGE; 10127; 111424; 113987; 6653Molecular structure and elemental composition of soybean (Glycine max L. Merr.) seeds of S04-05 (Ustun-1) variety together with its salt tolerant mutants were investigated by Fourier transform infrared (FTIR) and energy dispersive X-ray fluorescence (EDXRF) spectrometry. Salt tolerant soybean mutants were in vivo and in vitro selected from the M-2 generation of gamma irradiated S04-05 soybean variety. Examination of the secondary structure of proteins revealed the presence of some alterations in soybean mutants in comparison to those of the control groups. The difference IR spectra indicated that salt tolerant mutants (M-2) have less protein but more lipid contents. Chemometric treatment of the FTIR data was performed and principle component analysis (PCA) revealed clear difference between control group of seeds and mutants. EDXRF analysis showed that salt tolerant mutants considerably contained more chlorine, copper and zinc elements when compared to the control group, although most of the trace elements concentrations were not significantly altered. (C) 2013 Elsevier B.V. All rights reserved.Publication Metadata only Antioxidative defense system differences to drought stress of tomato cultivars(2014) AYAN, ALP; ATAK, ÇİMEN; ÇELİK, ÖZGE; 185510; 113987; 6653Publication Metadata only Induction and selection of salt tolerant soybean Glycine max L Merr Mutants(2012) Atok, Olgun; Erdoğmuş, Mehmet; ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987Publication Embargo Applications of Ionizing Radiation in Mutation Breeding(InTech, 2017-05) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653As a predicted result of increasing population worldwide, improvements in the breeding strategies in agriculture are valued as mandatory. The natural resources are limited, and due to the natural disasters like sudden and severe abiotic stress factors, excessive floods, etc., the production capacities are changed per year. In contrast, the yield potential should be significantly increased to cope with this problem. Despite rich genetic diversity, manipulation of the cultivars through alternative techniques such as mutation breeding becomes important. Radiation is proven as an effective method as a unique method to increase the genetic variability of the species. Gamma radiation is the most preferred physical mutagen by plant breeders. Several mutant varieties have been successfully introduced into commercial production by this method. Combinational use of in vitro tissue culture and mutation breeding methods makes a significant contribution to improve new crops. Large populations and the target mutations can be easily screened and identified by new methods. Marker assisted selection and advanced techniques such as microarray, next generation sequencing methods to detect a specific mutant in a large population will help to the plant breeders to use ionizing radiation efficiently in breeding programs.Publication Metadata only An investigation on regulation on trichome metabolism related genes against salt stress in soybean (Glycine max L. Merr.)(2014-06-25) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019Publication Metadata only Comparative transcriptional profiling of soybean orthologs of arabidopsis trichome developmental genes under salt stress(Springer, 233 Spring St, New York, Ny 10013 USA, 2018-02) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019The aim of this study was to determine the ultrastructural changes and regulation of trichome-metabolism-related genes against salt stress in soybean (Glycine max L. Merr.) plants. The 14-day-old Ataem-7 and S04-05 soybean seedlings were subjected to 0, 50, 100, and 150 mM NaCl stress. While the chlorophyll quantities were reduced, the activities of guaiacol peroxidase were increased in both varieties due to increasing NaCl concentrations. In S04-05 soybean variety, trichome densities were increased on both surfaces of the leaves whereas decreases were recorded in Ataem-7 variety at 150 mM NaCl treatment. Stomatal densities were increased on both surfaces of the leaves of both soybean varieties after salinity stress. We also performed a qRT-PCR analysis to evaluate the relative transcription levels of the soybean orthologs of Arabidopsis trichome developmental genes. qRT-PCR analysis demonstrated an induction of the soybean orthologs of GL2 and GL3 genes in soybean plants after 50, 100, and 150 mM NaCl treatments in both varieties. While the expression level of TTG1 ortholog gene was negatively affected in both soybean varieties under different concentrations of salinity, GL1 ortholog gene expression profile differed as a result of changing salt concentrations in both varieties with respect to control plants. It is observed that the regulation of trichome formation differs between two soybean varieties.