Person: ATAK, ÇİMEN
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ATAK
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ÇİMEN
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Publication Metadata only Radiosensitivity in callus tissues of soybean(glycine max.(L.)merril)(1994) Alikamanoğlu, Sema; ATAK, ÇİMEN; 6653; 176641Radiosensitivity of soybean as expressed by callus growth was examined after exposure of primary leaf explants with gamma rays.The leaf explants were irradiated 24 hours after incubation on the medium with gamma radiation doses of 0,5,7.5,10,25,50,75 and 100 Gy from Co-60 source. At the 30-day culture of callus the percentage of callus formation from irradiated explants was not different from the percentage of control callus formation. The decreasing average callus fresh weights were in connection with the increasing doses of the radiation and this relationship was significant (P<0.05).The differences between the doses of 5 Gy with 50 Gy and 100 Gy were significant (P<0.05) and the Gr-50 dose was found as 37 Gy for J-357 soybean variety.But the comparison of close doses of radiation was not significant from the point of decreasing average callus fresh weightPublication Metadata only Induced mutation and radiation sensitivity in vitro culture of soybean (Glycine max L. Merrill)(1999) Alikamanoğlu, S.; Yalçın, S.; ATAK, ÇİMEN; 6653; 176641In our research, the seeds of J-357 soybean variety were irradiated with 50, 100, 200, 300 and 500 Gy of gamma rays. Callus and meristem cultures were established from the primary leaves and apical meristem of 5-day-old seeding from the irradiation seeds. The average callus fresh weight formed at the cultures were determined on the 30th day and GR50 dose which reduces the callus fresh weight at the rate 50% in accordance with control was found as 250 Gy. The regenerated plants from meristem cultures were determined on the 28th day and LD50 dose which decreases the regenerated plant percentage at the 50% in accordance with control was found as 220 Gy. Cytological investigation was done at the callus and meristem cultures obtained from both irradiated seeds and explants. At the seed and explant irradiations, gamma radiation dose between the ranges 50 to 200 Gy and 5 to 25 Gy; respectively were used. Observations were done at mitotic anaphase in the samples taken from meristem and callus cultures. In anaphase, two types of cytological aberrations, bridge and fragments were determined. The regenerated plants were grown in the climate chamber and the seeds were taken from each M1 plant and chlorophyll mutants were determined in M2 generation. The radiosensitivity of the meristem cultures established by irradiated seeds from view of plant regenerations and plant number which is taken seed was evaluated by comparing with the results of meristem cultures established from the irradiated explant from the point of in vitro mutation studiesPublication Metadata only Mutagenic efficiency of gamma irradiation in two soybean varieties(1984) Özbek, N.; ATAK, ÇİMEN; 6653Efficiency of gamma irradiation on certain characteristics such as seedling height and dry weight of Amsoy-71 and Calland soybean varieties has been measured considering to use this mutagen effectively in mutation breeding. A greenhouse experiment was conducted using different doses of gamma irradiation (0 to 70 krad) and two soybean varieties. All treatments were carried out in 5 replications and consisted of 50 seeds. Seeds were sown in pots and plants were grown under climatically controlled conditions. Seedling height measurements were made 14 days after the emergence when the first leaf has stopped its growth and dry weights were measured after 5 weeks of growth. ED50 (Effective Dose) values were also calculated in order to find out the suitable irradiation doses to be used in mutation induction for both varieties. The results showed clearly that seedling height and dry weight were affected by γ-irradiation and, as compared with the control, both of them were reduced as the dose was increased with some differences between the varieties. The soybean varieties also showed differences in terms of ED50 (Effective Dose) values and this value was found for Amsoy-71 as 16 krad, whereas for Calland as 20 krad. These results were in agreement with the literature values.