Person: ATAK, ÇİMEN
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ATAK
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ÇİMEN
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Publication Metadata only Differential regulation of antioxidative gene expressions in response to salt stress in rice(International Congress on Transcriptomics 27-29 Temmuz 2015, 2015) Candar, Bilgin; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 195745; 6653Rice (Oryza sativa L.) is one of the most important crops because it is a nutritional source of more than one-third of the world population. Soil salinity is one of the most important abiotic stress factors that affect plant growth and productivity adversely. Rice growth and yield is also affected by salinity and at the seedling stage although it is known to be susceptible to salinity. Reactive oxygen species are induced by salt stress and some responsive mechanisms are evolved against to the detrimental effects caused by salt stress. Salinity response is especially controlled by obtaining homeostasis between antioxidative mechanisms and accumulation of reactive oxygen species (ROS) produced as a result of oxidative stress caused by salinity. It is known that combined expression profiles of antioxidative system enzymes may provide increased tolerance capacity. Therefore, the expression profiles of antioxidative enzymes in two different rice cultivars which have different salt sensitivities at four different salt stress conditions were determined. Salinity responses of two different rice varieties were investigated at seedling stage. Four different salinity treatments were then applied using Yoshida solution containing 0, 30, 90, 150 and 210 mM NaCl to the nutrient solution for seven days. At the end of the seven days, the leaves were harvested and stored at -20oC for further experiments. The salt stress responsive gene specific primers were amplified by designed primers due to the 3?-UTR regions of each of the following genes by aligning all available related genes in the databases of NCBI and KOME: Mn-SOD, Cu/Zn-SOD, Fe-SOD, Cytosolic APX, Thylakoid-bound APX, stromal APX, Cytosolic GR and CatA. Mn-SOD was consisted with the trend of variation in SOD activities of rice varieties. The expression patternof CAT A gene was markedly decreased compared to control in both rice varieties. Str-APX gene expression was up-regulated during salt stress treatments in both rice varieties. Transcript levels of Cyt-APX and Thy-APX were up-regulated in accordance with increasing salt stress in Osmanc?k-97 variety. The expression pattern of gene encoding enzyme Cyt-GR1 showed a gradual up-regulation as a response to subjected increasing NaCl stress in Mevl�tbey variety while only after 90 mM treatment, an up-regulation was observed for Osmanc?k variety. These data indicated that the antioxidative responses of salt tolerant and salt sensitive rice varieties are differentially regulated.Publication Metadata only An investigation on regulation on trichome metabolism related genes against salt stress in soybean (Glycine max L. Merr.)(2014-06-25) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019Publication Metadata only The Comparison of P5CS Gene Expressions of Two Soybean Varieties Under Salt Stress(2011 In Vitro Biology Meeting, 2011) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653Publication Embargo Evaluation of Proline Accumulation and Delta(1)-pyrroline-5-carboxylate Synthetase (P5CS) Gene Expression during Salinity Stress in Two Soybean (Glycine max L. Merr.) Varieties(Hard, Post-Office Box, 10-718 Olsztyn 5, Poland, 2012) ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653The aim of our study was to compare the effects of salt stress in two soybean (Glycine max L. Merr) varieties. Two soybean genotypes (Ataem-7 and Ustun-1) were grown under 0, 50, 100, and 150 mM NaCl treatments, and the leaves were harvested for lipid peroxidation analyses, proline content and P5CS gene expression levels. According to the results of lipid peroxidation analysis, Ataem-7 variety was found to be more sensitive than Ustun-1 variety for NaCl stress. Proline is an important osmolyte accumulated under environmental stresses. As a response to salinity, we determined their proline levels. Proline accumulation in Ataem-7 variety increased 1:39 fold in accordance with Ustun-1 variety at 150 mM NaCl treatment. Glycine max Delta(1)-pyrroline-5-carboxylate synthetase (GmP5CS) gene expression levels under 50, 100 and 150 mM NaCl stress were determined. When the GmP5CS gene expression level was gradually increased in Ustun-1 variety, the highest gene expression level for Ataem-7 was determined at 100 mM NaCl. The GmP5CS gene expression in Ustun-1 at 150 mM NaCl increased 2.93 fold compared with 100 mM treatment. When we evaluate the relation between proline accumulation and expression levels of GmP5CS gene, it is obvious that accumulations of proline in two soybean varieties are under control of different mechanisms in the presence of salinity.